Fig. 2
From: G-protein coupled receptor 64 (GPR64) acts as a tumor suppressor in endometrial cancer
Effect of GPR64 loss on cell growth in human endometrial cancer cells. a and b The expression of GPR64 mRNA and protein in Ishikawa (a) and HEC1A (b) cells transfected with non-targeting pool (NT) siRNA or GPR64 siRNA was examined by RT-qPCR and Western blot analysis, respectively. c and d Colony formation assay of Ishikawa (c) and HEC1A (d) cells transfected with NT siRNA or GPR64 siRNA. Samples from each treatment were transferred to flat-bottomed 24-well plates and incubated. Cells were fixed and stained with crystal violet. The average colony formation number was quantified with crystal violet stained cells. e and f Cell proliferation assay of Ishikawa (e) and HEC1A (f) cells transfected with NT siRNA or GPR64 siRNA. The results represent the mean ± SEM. *, p < 0.05; **, p < 0.01; and ***, p < 0.001