Fig. 4
Description and qRT-PCR evaluation of predicted transcript targets after miR-182 silencing. a Microarray analysis in MICOL-14 h-tert and MICOL-14tum cell lines showed upregulation of miR-182 target gene transcripts after miR-182 inhibition (positive logFC comparing anti-miR-182 vs anti-miR-NC). b qRT-PCR evaluation of the transcript expression levels of selected genes in MICOL-14h-tert and MICOL-14tum cell lines. Data analysis was performed by ΔΔCt method, and the control groups (NT and anti-miR-NC treated cells) were used as sample references in cell lines. Data were expressed as mean values ± SD of three independent experiments. nRQ, normalized Relative Quantity. *p < 0.05 **p < 0.01. c NABP1 levels were compared in a pool of primary CRC samples (T), in which increased miR-182 levels were known, and matched normal colon mucosa (N)