Fig. 2

Effect of anti-miR-182 treatment on apoptosis and cell cycle progression of MICOL-14^h-tert and MICOL-14^tum cell lines. a miR-182 silencing was associated with increased sensitivity of cells to apoptosis in both MICOL-14^h-tert and MICOL-14^tum cell lines, as determined by Annexin V/PI staining at different time points following treatment. The results of three independent experiments in triplicate were expressed as mean fold change ± SD over the baseline apoptosis. b Western blot analysis (left panel) of cleaved Caspase-3 and PARP in MICOL-14^h-tert and MICOL-14^tum cell lines non-transfected (NT) and transfected with anti-miR-182 or control vector (miR-NC). The right panel shows the densitometric analysis of the ratio between cleaved and total PARP. β-actin was used as a loading control. The WB image is representative of three independent experiments; mean values ± SD of 3 consecutive experiments are shown in the right panel. c The cell cycle analysis was performed in MICOL-14^h-tert and MICOL-14^tum cell lines 48 h after treatment using Ki67 and DAPI staining. The control populations (NT and anti-miR-NC cells) were used as references at each time point. *p < 0.05