Fig. 2

MALAT1 and TALAM1 regulate the metastatic potential of triple negative breast cancer cells. a Quantification of MALAT1 and TALAM1 expression levels by ssRT-qPCR after incubation with specific antisense oligonucleotides (LNA Gapmers, Exiqon) in MCF10A cells without TGF-β (n = 3 ± SD. Two-sided Student’s t-test was used for statistical analysis; * p < 0.05; ** p < 0.01). Actin-β was used as reference genes. b Representative images of RNA-FISH in MCF10A cells after the indicated treatments. Single molecule FISH probes from Stellaris® were used against MALAT1 (red). The corresponding images stained with DAPI are shown (scale bars correspond to 10 μm). c, d) Quantification of MALAT1 (c) and TALAM1 (d) expression levels by ssRT-qPCR after incubation with specific antisense oligonucleotides (LNA Gapmers, Exiqon) in MCF10A cells exposed to 10 ng/ml of TGF-β (n = 3 ± SD. Two-sided Student’s t-test was used for statistical analysis; *p < 0.05; **p < 0.01; ***p < 0.001). Actin-β was used as reference genes. e Representative images of RNA-FISH in MCF10A cells exposed to TGF-β after the indicated treatments. Single molecule FISH probes from Stellaris® were used against MALAT1 (red). The corresponding images stained with DAPI are shown (scale bars correspond to 10 μm). f-h Quantification of the levels of the metastatic markers GPC8 (f), CDCP1 (g) and MIA2 (h) by qPCR after incubation with specific antisense oligonucleotides (LNA Gapmers, Exiqon) in MCF10A cells exposed to TGF-β and in the triple-negative MDA-231 breast cancer cell line (n = 3 ± SD. Two-sided Student’s t-test was used for statistical analysis; **p < 0.01). Actin-β was used as reference genes