Fig. 1

MALAT1 and TALAM1 in breast cancer. a Strand-specific RT-qPCR was used for the quantification of MALAT1 and TALAM1 levels in different breast cancer cell lines. MCF10A cells (non-tumorigenic breast cells) were used as control. The results are presented as relative fold-level compared to the MALAT1 levels in MCF10A cells (n = 3 ± SD. Two-sided Student’s t-test was used for statistical analysis; ** p < 0.01; *** p < 0.001). Actin-β was used as reference gene. b Strand-specific RT-qPCR was used for the quantification of MALAT1 and TALAM1 levels in MCF10A cells exposed or not to TGF-β (10 ng/ml, for 24 h). The results are presented as relative fold-level compared to the MALAT1 levels in MCF10A cells (n = 3 ± SD. Two-sided Student’s t-test was used for statistical analysis; *** p < 0.001). Actin-β was used as reference gene. c Scheme of the MALAT1/TALAM1 locus with reference for the used primers (dark grey – MALAT1; light grey – TALAM1). d Strand-specific RT-qPCR was used for the quantification of MALAT1 and TALAM1 levels in different sub-cellular compartments of MCF10A cells exposed or not to TGF-β (10 ng/ml, for 24 h). The results are presented as relative fold-level compared to the MALAT1 levels in MCF10A cells (n = 3 ± SD. Two-sided Student’s t-test was used for statistical analysis; * p < 0.05). Actin-β was used as reference gene. e Representative images of RNA-FISH in MCF10A cells exposed or not to TGF-β (10 ng/ml, for 24 h) and in MDA-231 cells. Single molecule FISH probes from Stellaris® were used against MALAT1 (red). The corresponding images stained with DAPI are shown (scale bars correspond to 10 μm)