Fig. 5
NR2F1 overexpression inhibited the growth of SACC-83 cells and facilitated lung metastasis of SACC-83 cells in vivo. (a) Comparison of mice weights before and after injection with SACC cell showed a heavy loss of weight in NR2F1hi group. * p < 0.05. (b) The growth of nude mice bearing SACC. Tumor volumes were measured every 5 days and the data showed that tumor grew slowly when NR2F1 was overexpressed in SACC cells. *p < 0.05. (c) 3 of 6 mice were detected tumor metastasis in lung of NR2F1hi group (50%) and 1 of 6 mice were founded micro-metastasis of blank control group (16.7%) after 4 weeks. There was significantly difference between NR2F1hi and the control group. * p < 0.05. (d) Extraction the lung tissues from nude mice after 4 weeks and staining for HE showed that the blank control group has no significant tumor cells, while the NR2F1hi SACC group could detect tumor mass. Scale bar = 100 μm, SP × 100; Scale bar = 10 μm, SP × 400. (e) HE staining for tumor tissues which share the same pattern with human SACC tissues and was positive for PCK staining. No expression of NR2F1 was detected in blank control group and the positive expression of NR2F1 was showed in NR2F1hi group. Scale bar = 20 μm, SP × 400