Fig. 4
Effect of NR2F1 silencing on the dormancy, migration and invasion of SACC-83 and SACC-LM cells. (a) CCK8 assay was used to examine the cell growth rates in control, NR2F1hi SACC cells, NR2F1hiSACC cells + NR2F1 siRNA group of SACC-83 and SACC-LM, respectively. The data showed that NR2F1 siRNA can rescue the proliferation of cells. Error bars represent the mean ± SD of triplicate experiments. *p < 0.05. (b) Flow cytometry was used to examine the cell cycle in control, NR2F1hi SACC cells, NR2F1hiSACC cells + NR2F1 siRNA group of SACC-83 and SACC-LM, respectively. Representative figures of three independent experiments were shown.(c) Migration assay examined the cell migration ability in control, NR2F1hi SACC cells, NR2F1hiSACC cells + NR2F1 siRNA group of SACC-83 and SACC-LM, respectively. Representative figures of three independent experiments were shown. NR2F1 siRNA could reduce the migration ability of SACC cells, compared with NR2F1hiSACC cells. The mean was derived from cell counts of 3 fields, and each experiment was repeated 3 times. Error bars represent the mean ± SD of triplicate experiments. *p < 0.05. (d) Invasion assay examined the cell invasion ability in control, NR2F1hi SACC cells, NR2F1hiSACC cells + NR2F1 siRNA group of SACC-83 and SACC-LM, respectively. Representative figures of three independent experiments were shown. NR2F1 siRNA could inhibit the invasion ability of SACC cells compared with NR2F1hiSACC cells. The mean was derived from cell counts of 3 fields, and each experiment was repeated 3 times. Error bars represent the mean ± SD of triplicate experiments. *p < 0.05. (e) Flow cytometry showed cell apoptosis in control, NR2F1hi SACC cells, NR2F1hiSACC cells + NR2F1 siRNA group of SACC-83 and SACC-LM, respectively. Apoptotic analysis of SACC cells showed no difference in siNRF1 SACC cells and NR2F1hi SACC cells. Representative figures of three independent experiments were shown