Fig. 1

Characterization of patient-derived GSCs. Magnetic resonance imaging of four GBMs in the study cohort (a) and the corresponding xenografts (b) demonstrating that GSC cultures established from a heterogeneous GBM population display culture-to-culture heterogeneity in their in vivo formation characteristics. Images in (b) are stained with Hematoxylin & Eosin (h&e) in the upper image and Hoechst 33258 in the lower image. Tumor borders are macro-anatomically delineated. Scale bar 1 mm. (c) All histopathological features of glioblastoma were identified, including pathological angiogenesis (whole arrow), intratumoral hemorrhages (dotted arrow), tumor necrosis (triangle), pseudopalisading (asterisk) and nuclear atypia with aberrant mitoses. All tumors were xenografted to ≥2 mice. (d) Upon differentiation, the cells displayed a more mature morphology and stained positive for nestin and GFAP, however the individual GSC culture displayed intertumoral variability in their differentiation morphology. Scale bar 50 μm. (e) The cultures displayed variability in their capacity for total cell yield following serial passages, and (f) intertumoral heterogeneity in expression of stem cell markers (f). Expression of stem cell markers are data generated from n = 1 experiments in the individual cultures