Gene Symbol | Total exon fragments | FPKM |
|---|
LPS | SP 2/0 | LPS | SP 2/0 |
|---|
Pax5 | 11,625 | 70 | 34.34 | 0.71 |
Bach2 | 3318 | 0 | 7.92 | 0 |
Bcl6 | 403 | 230 | 4.25 | 5.59 |
Irf4 | 9191 | 5272 | 12.6 | 90.41 |
Prdm1 | 6055 | 1233 | 32.43 | 18.83 |
Xbp1 | 15,347 | 8127 | 196.23 | 293.8 |
Gm40600 | 552 | 4 | 22.87 | 0.35 |
- B cells from the splenocytes of three Balb/c mice per group were sorted by B220 microbeads, and stimulated for 3 days in vitro by 10 μg/ml LPS. SP 2/0 cells were thawed, passaged for 3 times and then cultured for 2 days in fresh medium. The transcripts in LPS-stimulated B cells and SP 2/0 cells were determined by RNA-sequencing. Total exon fragments and FPKM (FPKM = total exon fragments / mapped reads (millions) / exon length (KB), Fragments per Kilo bases per Million reads) values of selected genes encoding Pax5, Bach2, Bcl6, Irf4, Prdm1, Xbp1, and Gm40600 are shown
