Fig. 1

Gm40600 overexpression suppressed proliferation and promoted apoptosis of SP 2/0 cells. a Gm40600 overexpression suppressed SP 2/0 cell proliferation. Gm40600- or Vector-expressing SP 2/0 cells were cultured in vitro for 0, 1, and 3 days. CCK8 assay was used to analyze to cell proliferation. b-d Gm40600 overexpression affected SP 2/0 cell apoptosis but not cell cycle. Gm40600- or Vector-expressing SP 2/0 cells were cultured in vitro for 2 days. Cells were then stained and analyzed using propidium solution (PI) and flow cytometry (FACS), respectively. FACS profile, cell cycle, and apoptotic cell percentages are shown in (b, c, and d), respectively. d Two-tailed Student’s t test and a, and b two-way ANOVA followed by Bonferroni post-tests to compare each column to control column. Error bars, SEM. **p < 0.01, ****p < 0.0001