Fig. 2
HOXB-AS3 promotes cell proliferation in myeloid cells. a Proliferation assay of OCI/AML3 cells infected with lentivirus carrying pLKO-vector (control), shHOXB-AS3#1, or shHOXB-AS3#2. The infected cells were sorted by the expression of GFP. The sorted GFP-positive cells were seeded with a number of 1 × 106 in 5 mL culture medium on Day 0. Cell number was counted for 6 days. P values were calculated by Kruskal-Wallis test. b Quantitative PCR analysis of HOXB-AS3 relative to β2 microglobulin expression in OCI/AML3 cells stably carrying pLKO-vector (control), shHOXB-AS3#1, or shHOXB-AS3#2 by lentivirus infection. c The percentage of OCI/AML3 cells in the S phase of cell cycle. The result was derived from triple repeats. P values were calculated by Kruskal-Wallis test. d Quantitative PCR analysis of HOXB-AS3 relative to β2 microglobulin expression in TF-1 cells stably carrying pLKO-vector (control) or shHOXB-AS3#1 by lentivirus infection. e The percentage of TF-1 cells in the S phase of cell cycle. The result was derived from triple repeats. P values were calculated by Student t test. f Quantitative PCR analysis of HOXB-AS3 relative to β2 microglobulin expression in TF-1 cells infected with lentivirus carrying pLAS5w.Pbsd vector (control) or pLAS5w.Pbsd-HOXB-AS3 (HOXB-AS3 overexpression). g The percentage of cells in the S phase of cell cycle. The result was derived from the triple repeats. P values were calculated by Student t test. (* meant that P value was less than 0.05)