Fig. 3

Effect of PRAF2 on proliferation and cell cycle in ESCC cells. a Relative PRAF2 mRNA expression was determined using qPCR. b PRAF2 protein was detected by western blot in TE1 cells with PRAF2 silencing. The relative density of PRAF2 was quantified and normalized to GAPDH. c CCK-8 assay was performed to determine the ability of cell proliferation. c Colony formation assay was performed to determine the clonogenic capacity of TE1 cells. d The bar chart represented the number of colonies. e The cell cycle distribution was determined using flow cytometry. f The histogram of quantitative analysis in different mitotic phases. Values represented the mean ± SD data in triplicate. **P < 0.01