Fig. 1

Schematic workflow of glycoblotting-based MALDI-TOF/MS analysis of N-glycomes derived from whole serum and purified IgG fraction. The major steps include: a Purification of IgG fraction from whole serum using protein G resin, b confirmation of IgG by SDS-PAGE, C) Enzymatic release of glycans directly from serum or from IgG fraction, 1) Chemoselective capturing of reducing sugars onto a hydrazide-functionalized BlotGlycoH beads, 2) washing to remove all impurities, 3) On-bead methyl esterification of sialic acid residues, 4) Recovery of BOA-labeled glycans, 5) MALD-TOF/MS and data analysis