Fig. 8

The regulatory role of DNMT1 siRNA on hsa-miR-124-3p / BCAT1 in ESCC cells. a The methylation status of hsa-miR-124-3p in KYSE-150 and Eca109 cells transfected with DNMT1 siRNA or NC siRNA was detected by methylation specific PCR. b The mRNA expression of DNMT1, hsa-miR-124-3p and BCAT1 in KYSE-150 and Eca109 cells transfected with DNMT1 siRNA or NC siRNA were determined by qRT-PCR analysis. c-d The protein expression of DNMT1 and BCAT1 were detected by western blotting assay (c) and immunofluorescence staining assay (d) respectively. The target protein expression relative to GAPDH expression was displayed on the right of Fig. 8c. The experiments were performed in triplicate and each value represents mean ± SD. ^***P < 0.001