Fig. 2

TSPAN9 affects gastric cancer migration and invasion. a Following a 48 h 10 ng/ml TGF-β1 treatment, TSPAN9 expression was assessed. b qRT-PCR was used to quantify EMT-related gene expression following TSPAN9 siRNA transfection (or control transfection). c Western blotting was used to assess levels of proteins linked with the EMT following siRNA transfection. After TSPAN9 siRNA transfection, wound-healing (d) and migration assays (e) examined how this siRNA affected GC cell migration. A total of 2 × 105 cells SGC7901 cells, si-RNA transfected subclones, and controls were used in a Transwell-based invasion assay. A total of 10 fields per insert were counted to determine the number of invading cells. n = 5 replicates. *p < 0.05, **p < 0.01, Student’s t test