Fig. 3

S100A8/S100A9 induction accompanied by inactivation of YAP and activation of Hippo pathway. Western blot analyses of S100A8/S100A9 and Hippo pathway in HCC94 (a and b) and FaDu (e and f) cells. Cells cultured in suspension for 2 days (S48 h) and then reattachment for 1 day (S48 h-reattached). Cells cultured densely for 2 days (D48h) and then relief from dense culture (D48h-sparse). GAPDH was used as a loading control. The expression of S100A8, S100A9, CTGF and CYR61 were analyzed by qPCR in HCC94 cells (c and d) and FaDu cells (g and h). Error bar, SD of three different experiments. *p < 0.05, **p < 0.01; t-test