Table 3 Primer sequences for quantitative reverse transcription polymerase chain reaction
Gene | Forward primer | Reverse primer |
|---|---|---|
lnc-LHFPL3–5 | 5′ ACTTCTACTATTGATGCAGTCC 3’ | 5′ AAGTTCAGAACTGACTGTCTAT 3’ |
lnc-GUCY1A3–1 | 5′ GCATCCAGACACTGCTTAATC 3’ | 5′ GCAAGGAGGTGAAGGAATAG 3’ |
lnc-AL445665.1–4 | 5′ ATTCTGATTCCCTGTGTATCCA 3’ | 5′ CTGTGACAGAAATAGGAATGCC 3’ |
lnc-RP4-725G10.1.1–8 | 5′ GCTCAGGACTTTCACTGATT 3’ | 5′ GTTGTTTCAACTTCTTTCCCAC 3’ |
ACTB | 5′ CCATCATGAAGTGTGACG 3’ | 5′ GCCGATCCACACGGAGTA 3’ |
miR-146b-5p | 5′ GCGCAGTGAGAACTGAATTCCA 3’ | 5′ AGTGCGTGTCGTGGAGTCG3’ |
U6 | 5′ CGATACAGAGAAGATTAGCATGGC 3’ | 5′ AACGCTTCACGAATTTGCGT 3’ |