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Fig. 4 | BMC Cancer

Fig. 4

From: Programmed expression of pro-apoptotic BMCC1 during apoptosis, triggered by DNA damage in neuroblastoma cells

Fig. 4

E2F1 is necessary for induction of BMCC1 with CDDP treatment. a Knockdown of E2F1 stops the accumulation of BMCC1 and phospho-ATM (p-ATM, ATM-S1981) in response to CDDP treatment. E2F1-depleted and non-depleted SK-N-AS cells were exposed to CDDP. Cell lysates were extracted at regular time intervals and analyzed by immunoblotting with the indicated antibodies. b Silencing of E2F1 diminishes CDDP-dependent activation of BMCC1 promoter. E2F1-depleted and non-depleted SK-N-AS cells were transfected with pGL3-BMCC1-luc. Forty-eight hours after transfection, the cells were treated with CDDP or left untreated. Two hours after treatment, the activity of luciferase was measured for all samples. Data are shown as means ± SD (*P < 0.01, n = 3)

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BMC Cancer

ISSN: 1471-2407

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