Fig. 2

Knockdown of SNX27 reduced the proliferation and migration of MDA-MB-231 breast cancer cells. a Proliferation of SNX27-KD MDA-MB-231 cells was significantly decreased comparing with WT control MDA-MB-231 cells by MTT assay conducted at time points 24, 48, and 72 h. Triplicate samples of each wafer from three independent experiments were included at each of the 3 time points (n = 3). Data are analyzed using generalized linear mixed models, and expressed as mean ± SD. *p < 0.05 vs. WT control; **p < 0.01 vs. WT control. b and c Wound healing analysis of SNX27-KD and WT MDA-MB-231 cells showed that the WT cancer cells can recover ~ 50% of the wound area in 8 h and heal the whole area in 18 h, but KD cells took 3 h longer than WT cancer cells to cover the whole wound area. Meanwhile, the percentages of healing area were showed with different time points. The migration rate was expressed as a percentage of scratch closure. The values are the means of three independent wound fields in three repeated time. Data are analyzed using generalized linear mixed models, and expressed as mean ± SD from three independent experiments (n = 3). *p < 0.05 vs. WT control; **p < 0.01 vs. WT control. Scale bar is 200 μm