Fig. 2
H2A.X Y142ph restrains SNU-16 cell growth. SNU-16 cells were transfected with pEGFP-N1, pEGFP-H2A.X, pEGFPH-RasG12V/T35S, or pEGFP-H2A.XY142A (indicated as GFP, H2A.X, RasG12V/T35S and H2A.X Y142A, respectively) plasmids. a The expression of H2A.X Y142ph levels were detected by western blot. b Cell viability, (c) numbers of colonies and (d) cell migration were detected by MTT assay, soft agar, and Transwell assays, respectively in SNU-16 cells. (e) The expression of H2A.X Y142ph levels were detected by western blot. f Cell viability, (g) numbers of colonies and (h) cell migration were detected by MTT assay, soft agar, and Transwell assays, respectively in MKN1 cells. Data presented as mean + SD, * P < 0.05, ** P < 0.01, *** P < 0.001 (n = 3)