Fig. 2

miR-188-5p promotes migration, invasion, and metastasis of GC cells. a GSEA plot showing miR-188-5p expression in association with metastasis-related gene signatures. The miR-188-5p level was positively correlated with activated metastasis-related gene signatures and inversely correlated with suppressed metastasis-related gene signatures in the TCGA stomach cancer dataset. b A wound-healing assay was performed with the indicated cells; Scale bar, 400 μm. c Representative images and quantification of Transwell assays in the indicated cells; Scale bar, 200 μm. d BALB/c nude mice injected intravenously via the caudal vein with indicated cells were monitored with a luciferase live-imaging system at indicated time points. Heat map scale bar represents photon emission. e Representative bright-field imaging of the lungs on 50 days of mice (left); H&E staining of lung metastases formed by the indicated cells (middle) and number of visible surface metastatic lesions in mice (n = 5 per group) injected intravenously with indicated cells (right), Scale bar, 200 μm. Error bars represent mean ± SD from three independent experiments. f Survival curves of mice injected intravenously with indicated cells. Error bars represent mean ± SD (n = 5 per group). **, P < 0.01. GC gastric cancer; GSEA, gene set enrichment analysis; H&E, hematoxylin and eosin; NC, normal control; TCGA, The Cancer Genome Atlas