Fig. 3
From: ERO1α is a novel endogenous marker of hypoxia in human cancer cell lines
Immunohistochemical analysis of ERO1α and CA9 in cultured cells. HeLa (a) or HCT116 (b) cells were cultured until sub-confluent, collected using iPGell, formalin-fixed, paraffin-sectioned, and stained. Representative haematoxylin and eosin (HE, left), ERO1α (middle), and CA9 (right) staining images of cells grown under normoxia (upper panel) and hypoxia (lower panel) are shown. Scale bar: 50 μm