Fig. 1
From: ERO1α is a novel endogenous marker of hypoxia in human cancer cell lines
Expression of ERO1α and CA9 in human cell lines. a Endoplasmic reticulum disulphide oxidase 1α (ERO1α) and carbonic anhydrase 9 (CA9) protein expression in normal (NHDF and HEK293) cell lines and cells lines from different cancer types: colon (HCT116, SW480, and HT-29); breast (MDA-MB-231 and MCF7); cervical (HeLa); lung (A549); pancreatic (MIAPaCa2 and PANC1); liver (HepG2); and fibrosarcoma (HT-1080). The cells were analysed by western blotting (WB). Quantified values of ERO1α (left graph) and CA9 (right graph) are indicated. The values were normalised to those of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The values obtained from NHDFcells were set to 1.0 and are given as the mean ± the standard error of the mean (SEM) (b) Gene expression analysis of ERO1α and CA9 by quantitative PCR (qPCR) in the above-mentioned cell lines. The relative amounts of mRNA were calculated from the comparative threshold cycle (Ct) values relative to those of GAPDH. Data are presented as the mean ± the standard error of the mean (SEM)