Fig. 5

Expression of HOXA5 short RNA in cancer cell lines and colon cancer tissues. a. HOXA5 short RNA expression in normal colonic epithelial (HCEC-1CT), colorectal cancer cells (HCT116, DLD1 and HT-29), normal bronchial epithelial (BEAS-2B), and lung cancer (A549) cells was measured by qPCR using GAPDH mRNA as an endogenous quantitative control. Data are expressed as the mean fold changes ± standard deviation (SD; n = 4) compared with those in the HCEC-1CT cells or BEAS-2B. (*P < 0.05, Dunnett’s test (colon) or unpaired Student’s t-test (lung)). b, c, and d. The expression of HOXA5 short RNA (b) and HOXA5 mRNA (c) in cDNA libraries prepared from colon cancers and surrounding normal colonic mucosa from 23 patients were measured by qPCR. ACTB mRNA was used as an endogenous quantitative control. Relative expression of HOXA5 short RNA to HOXA5 mRNA are shown in d. *P values were calculated by the paired Student’s t-test. NS = not statistically significant difference