Fig. 4
From: A novel long non-coding RNA from the HOXA6-HOXA5 locus facilitates colon cancer cell growth
HOXA5 short RNA activated epidermal growth factor (EGF) signaling. a and b. Gene expression profiles in HOXA5 short RNA-overexpressing cells and silencing cells (siRNA #7 and siRNA #8) were analyzed using a whole human genome microarray (Agilent Technologies) and GeneSpring 14.9 (Agilent Technologies). IPA analysis of the top-five ranked bio-functions (a) and predicted upstream regulators (b) for the differentially expressed genes between the two types of cells. c and d. Protein levels of phosphorylated EGF receptor (EFGR) and total EGFR were measured by western blot analysis. GAPDH levels were used as an endogenous quantitative control. The level of phospho-EGFR or EGFR band relative to that of GAPDH was quantitatively analyzed by densitometry as indicated