Fig. 3

Regulation of cell proliferation and migration by stably overexpressed HOXA5 short RNA. After HCT116 (a), DLD1 (c), and HT-29 (e) cells were transfected with pEB-HOXA5 short or pEB-mock vector and selected using blasticidin, expression levels of HOXA5 short RNA and HOXA5 mRNA were measured by qPCR using GAPDH mRNA as an endogenous quantitative control. Data are expressed as the mean fold changes ± standard deviation (SD; n = 4) compared with those in the pEB-mock cells. *Statistically significantly difference versus the pEB-mock cells (unpaired Student’s t-test, P < 0.01). b, d, and f. The indicated cells (1.0 × 10⁵ cells) were seeded into 35-mm-diameter dishes and the growing cells were counted at the indicated times. Values are means ± SD (n = 4). *Statistically significantly difference versus the pEB-mock cells (unpaired Student’s t-test, P < 0.01). g. After the indicated cells were cultured with serum-free medium for 36 h, they were seeded in serum-free medium onto the upper side of a Transwell chamber and allowed to migrate towards 10% FBS-containing medium in the lower chamber. After incubation for 24 h, the migrating cells were stained with Diff-Quick dye (lower panels) and counted (upper panel). Data are presented as the means ± SD (n = 4). *P < 0.05, unpaired Student’s t-test