Fig. 8

Imaged-based cell fate assay enables measurement of RMIC on melanoma cells in co-culture with fibroblasts. Figure panels in (a) show all cells labeled with Hoechst (left), fibroblasts labeled with H2B-eGFP (middle) and dead cells labeled with EtHd (right), with the merged image of all three labels shown in (b). Plot in panel (c) shows the quantification of melanoma and fibroblast viability in co-culture resolved by computational image analysis, compared to melanoma cells in monoculture. Data shown are three independent experimental repeats with results presented as average ± SEM. (* p value < 0.05)