Fig. 7

Image-based analysis of RMIC treatment in spheroid models. a Example images of A375 melanoma spheroids generated in 3D collagen. Example images of spheroids imaged using wide-field epifluorescence (b) and LSFM (c). d Aggregate data comparing the two imaging platforms with similarly treated single cells cultured in 3D collagen from three independent experimental repeats with results presented as average ± SEM, normalized to DMSO control. e Examples of proliferation heterogeneity in spheroids created from single cells. Of the 42 spheroids imaged, 35 showed no proliferation, 4 spheroids showed low proliferation and 3 spheroids showed high proliferation. Data for the 42 spheroids were a collection from three independent experimental repeats