Fig. 2

Cell fate assay is capable of identifying differences in proliferation and viability in 2D and 3D microenvironments. Example images show maximum intensity projections of 3D Z-stacks of A375 cells stained with viability markers (a) and a proliferation marker (b) cultured in 3D collagen and treated with various concentrations of the RAF/MEK inhibitor combination. Plots show aggregate viability score (c), proliferation score (d), and Cell Titer-Glo assay readout (e) normalized to DMSO controls, for A375 cells cultured either in 2D dishes or 3D collagen cultures for three independent experimental repeats. Results are shown as average ± SEM (*p < 0.005, ** p < 0.0005, *** p < 0.00005)