Fig. 1

Image-based proliferation and viability measurement of drug-treated cells in 2D and 3D samples. a Cell viability is measured by image-based quantification of cells positive for extracellular phosphatidyl serine (apopxin) or disrupted plasma membrane (EtHd) and proliferation is measured by image-based quantification of cells positive for EdU incorporation over a 24 h period. b. Quantification in 3D samples is performed by obtaining a 3D image stack, analyzing each image slice separately and aggregating data from all image slices. c Effects of RAF/MEK combination treatment on melanoma cells cultured in standard 2D polystyrene dishes were compared with cells cultured in 3D collagen. d RMIC treatment targets mutant BRaf (Dab) Raf and wild type Mek in the MAP kinase pathway