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Fig. 3 | BMC Cancer

Fig. 3

From: Raman spectroscopy detects metabolic signatures of radiation response and hypoxic fluctuations in non-small cell lung cancer

Fig. 3

Concurrent immunofluorescence staining of γ-H2AX associated DNA damage, and hypoxia indicated through pimonidazole. Immunofluorescence images of 0 Gy (left panels) and 15 Gy (right panels) tumours harvested at 2 h (a, b), 3 (c, d) and 10 (e, f) days post irradiation. Nuclear localization was made using DAPI (blue), while DNA damage was indicated through γ-H2AX (red) and hypoxia through pimonidazole (green)

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