Fig. 4

PTEN inhibition results in increased expression of EMT-associated genes expression. a Western blot analyses showing the protein level of PTEN, FAK, p-FAK and EMT-related genes (N-cadherin, E-cadherin, Snail-1, MMP-9) in mock, Numb-overexpressed, Numb-overexpressed plus VO-Ohpic trihydrat-treated SCC-9 and CAL-27 cells. GAPDH serves as internal control. b Gray level analysis of the protein level of PTEN, FAK, p-FAK and EMT-related genes (N-cadherin, E-cadherin, Snail-1, MMP-9) in mock, Numb-overexpressed, Numb-overexpressed plus VO-Ohpic trihydrat-treated SCC-9 and Cal-27 cells after western blot. The mean ± SD represents the average levels from 3 replicates. *p < 0.05, **p < 0.01, ***p < 0.001