Fig. 2
From: Salinomycin triggers endoplasmic reticulum stress through ATP2A3 upregulation in PC-3 cells
ATP2A3 overexpression triggered ER stress. a. Salinomycin induced ATP2A3 upregulation in PC-3 and DU145 cells. b. Flow cytometry showed that ATP2A3 overexpression induced cell-cycle arrest in PC-3 and DU145 cells. *P < 0.05, vs. empty vector. c. Western blotting showed that ATP2A3 overexpression changed cell-cycle regulatory protein expression levels. d. Annexin V-FITC/PI staining showed that ATP2A3 overexpression induced PC-3 and DU145 cells apoptosis. *P < 0.05, vs. empty vector. e. Western blotting showed that ATP2A3 overexpression induced cleavage of PARP. f. ATP2A3 overexpression triggered ER stress in PC-3 and DU145 cells. Western blotting analysis of BIP, CHOP, Caspase 12, ATF4 expression and PERK, eIF2a phosphorylation level. g. Immumohistochemical staining showed that ATP2A3 and ER stress biomarkers (BIP and ATF4) expression in prostate cancer and para-carcinoma tissues. Scale bars, 10 μm