Fig. 3

Mifepristone reduces the migration of cancer cells of the ovary (a, b), breast (c), glia (d), or prostate (e) in a wound healing assay using fluorescent stains. Each cell line was treated with their respective concentration of MF for 72 h prior to wounding. Multiple images were taken of each wound, and the average wound width was calculated at 0 and 24 h. The width of the wound was measured four times per image. (b) Is a visual representation of SKOV-3 cells in a wound healing assay, labeled with Alexa Fluor®-594 Phalloidin and DAPI, to stain the cytoskeleton and nucleus respectively. Scale bar = 1000 μm. Data in a, c, d, and e represent the mean ± s.e.m. Vehicle (closed bars); MF (open bars). *P < 0.05, **P < 0.01, ***P < 0.001 compared against vehicle. Statistical analysis was done using two-way ANOVA followed by Bonferroni’s multiple-comparison test