Fig. 2

Phenotype of MNRR1 knockout cells and histochemistry. a Intact cellular oxygen consumption in HEK293 MNRR1-knockout (R1-KO) cells compared to controls harboring Wild Type (WT) MNRR1. **, p < 0.005. Inset shows an immunoblot confirming R1-KO phenotype. b Live staining of 293 WT and R1-KO cellular mitochondria using MitoTracker Green. Intensity of staining was calculated using ImageJ and has been shown in arbitrary units (A.U.). *, p < 0.05. c Immunohistochemistry of human invasive (IDC) (n = 4) and benign breast tissue (n = 3) stained with MNRR1. ImageJ quantitation of the staining intensity, described in Methods, is also shown. **, p < 0.005