Fig. 3

Death of TNF-treated MCF7 cells correlates with the processing of Poly (ADP-Ribose) Polymerase-1 (PARP1). a Western blot immunoassay of 30 μg of total proteins from MCF7 cells treated with ETOH, TNF, CORT + TNF or DEX + TNF for 24 h or 48 h (lanes 2–9). In lane 1, ultraviolet light (UV)-treated HeLa cells were used as a positive control for PARP1 processing; mature PARP1 (116 kDa) and cleaved PARP1 (89 kDa). b The cleaved PARP1/β-actin ratios of UV light-treated HeLa cells (lane 1) and of MCF7 cells treated with TNF for 48 h (lane 7) are shown. Data were normalized to the HeLa cell death model and are presented as the mean ± standard deviation (SD); n = 3