Fig. 3
From: Changes of N6-methyladenosine modulators promote breast cancer progression
Induction of m6A inhibited BC growth and metastasis. (a, b) Stable Overexpression of METTL14 by pcDNA3/Flag-METTL14 and/or shRNA knockdown of ALKBH5 in MDA-MB-231 cells was verified by qRT-PCR assay. Overexpression of METTL14 and/or knockdown of METTL3 significantly induced global m6A level by ELISA assay (c) but reduced cell viability by CKK8 assay (d), colony formation ability (e) and cell migration by transwell assay (f). (g) Representative images of colony formation and cell migration cells. * P < 0.05, ** P < 0.01, *** P < 0.001