Fig. 4

Flow-cytometric analyses of the sub-G₁ DNA content distribution in DK-MG and SNB19 cells subjected to drug-IR treatments were performed 30 min or 24 h after IR. The cells were detached with trypsin, treated with saponin and RNAse, stained with propidium iodide and then analyzed for red fluorescence by flow cytometry. The samples include both floating and trypsinized cells. Changes in the sub-G₁ fraction are depicted as folds of increase (means ± SE), normalized to respective non-irradiated drug-free controls (30 min post-IR). The sub-G₁ fraction includes hypodiploid nuclei and debris, computed from the flow cytograms with the Flowing Software for n = 3 independent experiments