Fig. 1

Impact of Akt and/or PI3K/mTOR inhibition on the migratory behavior of DK-MG and SNB19 cells assessed via single-cell tracking (a, b) and wound healing (c, d) tests. Part A illustrates migration paths of 20–30 representative cells during 5 h, for each treatment. For each cell, its initial position was set to the origin of coordinate system. Black circles denote the final cell locations at time = 5 h. The corresponding time lapse videos are given in Additional file 3: movie S1. Part b summarizes the mean (± SE) migration speed values (i.e. total distance, TD divided by time) from at least 400 cells per treatment. The bar graphs in C depict the impact of inhibitor treatment on the wound closure rate in DK-MG and SNB19 cell monolayers, expressed in unit area per min [μm²/min]. Each bar represents the mean ± SE of at least three independent experiments, each performed four times. Phase contrast images of GBM cells shown in part d were acquired at 0 and 6 h after the wound was made. White color in the right-side column of d represents the cell-devoid area, black and grey colors show areas covered with cells at time = 0 and 6 h, respectively. “*” stands for p < 0.05. “n.s.” means “not significant”