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Table 5 Comparison of genetic variants identified by target NGS in LBC and FFPE samples of thyroid tumors

From: Clinical impact of a cytological screening system using cyclin D1 immunostaining and genomic analysis for the diagnosis of thyroid nodules

Patient no Gene cDNA change Amino acid change Variant frequency (%) Read depth Functional consequence
Case 29 LBC HRAS c.81A > G   50 104 synonymous variant
TP53 c.215G > C p.72P/R 99.66 1468 missense variant
Case 29 FFPE HRAS c.81A > G   48.75 80 synonymous variant
TP53 c.215G > C p.72P/R 98.86 612 missense variant
Case 30 LBC BRAF c.1799A > T p.600 V/E 31.98 172 missense variant
Case 30 FFPE BRAF c.1799A > T p.600 V/E 2.87 348 missense variant
Case 31 LBC BRAF c.1799A > T p.600 V/E 40.46 351 missense variant
Case 31 FFPE BRAF c.1799A > T p.600 V/E 25.68 183 missense variant
  1. LBC liquid based cytology sample, FFPE formalin fixed paraffin embedded specimen