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Table 1 A statistical analysis of serum content and the muscle key proteins of a protein synthesis pathway

From: A leucine-rich diet modulates the mTOR cell signalling pathway in the gastrocnemius muscle under different Walker-256 tumour growth conditions

 

7th day

14th day

21st day

Serum Insulin profile

n.s.

*W < C

*W < C

*L > C

*W < WL

Serum Walker Factor

n.d.

n.d.

*W > C

*W > L

*W > WL; P = 0.0517

Phospho IRS-1/total IRS-1 ratio

*W < C

*W < C; P = 0.056

n.s.

Phospho AKT-PKB

n.s.

*WL < L

*W > C

*W > L

*WL < W

Phospho ERK-MAPK

n.s.

n.s.

*W > C

*W > L

Phospho mTOR

n.s.

n.s.

*WL > C

*WL > L

*WL > W

Phospho p70S6K

n.s.

n.s.

n.s.

pmTOR/p70S6K-1 ratio

*L > C

*WL < C

n.s.

*W < C

*WL > C,L

*WL > W

Phospho RAG-A

*L > C

*WL > C

*WL > L

*L > C

*W > C

*WL > C

*L > C

*WL > C

*W < L

*WL > W

Phospho JNK

*W > WL

n.s.

n.s.

Phospho STAT3

n.s.

n.s.

*W > C

Phospho STAT6

n.s.

*W > C

*W > C

*W > L

Phenylalanine Incorporation

n.d.

n.d.

*C > W

*L > WL

*WL > W

Leucine Incorporation

n.d.

n.d.

*W < C; p = 0,058

KIC Incorporation

n.d.

n.d.

*C > W

*WL > W

Tyrosine release

n.d.

n.d.

*W > C

*WL > L

*W > WL

Total protein net

n.d.

n.d.

*C > W

*L > WL

*WL > W

  1. Legend: C, control group; W, Walker tumour-bearing group; L, rats fed a leucine-rich diet; WL, tumour-bearing rats fed a leucine-rich diet. Two Way ANOVA analysis followed by the Fisher’s LSD multiple-comparison test (P < 0.05 values). * The mean difference of 0.05 is statistically significant. n.s. = non-significant. n.d. = non-determined. For details, see the Materials and Methods and Results sections