Fig. 1

Cell viability, measured in MTT-assay in Kelly (a), IMR-5 (b), SKNAS (c) and SY5Y (d) cells 72 h after treatment with variable concentrations of TW-37. The IC-50 value was determined for each cell line. e Western Blot of whole cell lysate of four neuroblastoma cell lines with antibodies against Bcl-2 and Mcl-1 protein, and the housekeeping protein β-actin. f SKNAS, SY5Y, IMR5 and Kelly cells were treated with 1 μM TW-37 following cell cycle analysis by FACS. Diagrammed is the percentage of cells in the different cell cycles. g Apoptosis was measured in SKNAS, SY5Y, IMR5 and Kelly cells after treatment with 1 μM TW-37. The enrichment factor was used as a parameter of apoptosis. h Proliferation SKNAS, SY5Y, IMR5 and Kelly cells after treatment with 1 μM TW-37 was measured by ELISA. The proliferation rate is given as a percentage of control