Fig. 2

Swainsonine restrained U251 cells proliferation and evoked apoptosis. a The normal astrocyte NHA cells were disposed with diverse dosages of Swainsonine (0–40 μM) for 12 h, cell viability was appraised by utilizing Cell Counting Kit-8 (CCK-8); U251 cells were administrated with diverse dosages of Swainsonine (0–40 μM) for 12 h. b Cell viability, c cell proliferation, d cell cycle and (e and f) CyclinD1, Cyclin-dependent kinase 4 (CDK4), and p16 were appraised by exploiting CCK-8, Bromodeoxyuridine (BrdU), flow cytometry and western blot; g cell apoptosis and (h) pro-Caspase-3, pro-Caspase-9, cleaved-Caspase-3 and cleaved-Caspase-9 were assessed by flow cytometry and western blot. *p < 0.05; **p < 0.01; ***p < 0.001; ns: no significance.