Fig. 7

BrMC inhibited MHCC97H sphere formation capacity induced by co-culture. a Western blot analyzed effects of BrMC and chrysin on the expression of FAP-α of LX-2 cells co-cultured with MHCC97H-derived LCSLCs, β-actin was used as loading control(left). Densitometry analysis quantify the expression of α-SMA and FAP-α respectively (^* P < 0.05 vs treated with 0.1% DMSO; ^# P < 0.05 vs treated with 20 μmol/L chrysin) (right). b. Effects of BrMC and chrysin on concentrations of IL-6 (left) and HGF (right) in Co-CM (^* P < 0.05 vs treated with 0.1% DMSO; ^# P < 0.05 vs treated with 20 μmol/L chrysin). c. Representative images of sphere forming cells in SMMC-7721 cells induced by Co-CM treated with BrMC and Chrysin (left). Effects of BrMC and Chrysin on sphere forming rate of SMMC-7721 cells induced by Co-CM (^* P < 0.05 vs treated with 0.1% DMSO; ^# P < 0.05 vs treated with 20 μmol/L chrysin)(right). d Western blot analyzed effects of BrMC and chrysin on the expressions of CD133 and CD44 of SMMC-7721 cells induced by Co-CM treated with BrMC and Chrysin, β-actin was used as loading control(left). Densitometry analysis quantify the expression of CD133 and CD44 respectively (^* P < 0.05 vs treated with 0.1% DMSO;^# P < 0.05 vs treated with 20 μmol/L chrysin) (right)