Fig. 4
From: Isocytosine deaminase Vcz as a novel tool for the prodrug cancer therapy
5-FIC cytotoxicity test of Vcz-expressing Caco-2 and U87MG cell lines. The cells were incubated with media collected from 293H cells, which were transfected with vectors pcDNA4/TO or pTO/Vcz-IG and treated with 100 μM 5-FIC or DMSO for 48 h. The growth media from treated control (designated as Ctrl. Medium) and Vcz-expressing cells (designated as Vcz Medium) was collected and applied onto a Caco-2 and b U87 MG cells. The calculations of Relative Absorbance values of treatment groups were carried out in the same way as for the Fig. 1. Statistical significance indicated by p-values, where the asterisk symbol (*) designates p < 0.0001 with respect to cell transfected with pcDNA4/TO and treated with DMSO