Fig. 7
From: Drug resistance profiling of a new triple negative breast cancer patient-derived xenograft model
Differential response of TU-BcX-2 K1 to HDAC inhibitors in 2D and 3D culture conditions. TU-BcX-2 K1 cells treated with the HDAC inhibitors panobinostat (panobin), romidepsin (romi) and vorinostat (vorin). Cell viability and morphology were visualized using a crystal violet staining in 2D conditions, b live/dead fluorescence staining in 2D conditions and c live/dead staining in 3D conditions. In the live/dead staining, live cells are stained with Calcein-AM (green), dead cells stained with EthD-III (red). Images were captured at 100X magnification. d Morphology-related gene changes after adherent TU-BcX-2 K1 cells treated with panobinostat, romidepsin and vorinostat. qRT-PCR was used to evaluate gene expression and results were normalized to β-actin and DMSO vehicle controls. Significance is represented as follows: * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent S.E.M. N = 3