Fig. 5

The role of CTR in metabolism, proliferation and patient outcome. a metabolic activity of SB2b was assessed by an MTT assay in the presence (+GF) or absence (−GF) of defined growth factors (EGF + bFGF) in combination with 1 μM of CTR agonists sCT or hCT or the CTR specific antagonist sCT(8–32), results are mean + S.E.M. of 3 independent experiments performed in triplicate. In b, the effect of 1 μM CTR ligands on cell proliferation was assessed using live cell imaging (Operetta) over 72 h, cell number per field is expressed as a Log₂ value and for clarity only every 3rd data point is shown, with representative experiment (of 3 independent experiments). Kaplan-Meier plots were derived from publicly available data using a threshold for expression of 1.25 FKPM (~ 1 transcript per cell) for all patients for whom RNAseq and survival data was available, CALCR +ve means over 1.25 FKPM, CALCR -ve means less than 1.25 FKPM, in c is the data from the IVY-GAP database and d shows data from TCGA