Fig. 4

EGF helps in PD-L1 transport to cell surface. a, shows that after EGF treatment the PD-L1 levels on cell surface increases analysed by flow cytometry analysis, compared to isotype control. Together with insulin EGF treatment can further increase the level. b, shows the MFI of repeated flow cytometry analysis. EGF treatment alone the MFI increased 44.7%, compared to isotype control. When co-treated with insulin, the MFI of EGF treatment further increased 20.4%, suggesting that EGF treatment is associated with membrane PD-L1 levels. c, shows the WB assay for PD-L1 in the cell membrane isolated from HT-29 cells treated with insulin or EGF+ insulin