Table 1 Demographic data of 29 patients and next-generation sequencing results of different samples
patient ID | Age(years) | Sex | Mutations detected by NGS in different Sample types (mutation allele fractions) | EGFR status in primary tumor by ARMS | |||
|---|---|---|---|---|---|---|---|
plasma ctDNA | CSF cfDNA | CSF cell | FFPE of primary cancer | ||||
#1 | 59 | M | EGFR L747_E749del (42.5%) | EGFR L747_E749del (98.1%) | EGFR L747_E749del (37.8%) | EGFR L747_E749del (29.2%) | EGFR wild-type |
#2 | 54 | F | No mutation | ALK G689R (1.0%) | No mutation | EGFR L858R (18.8%) | EGFR L858R |
#3 | 70 | M | No mutation | No mutation | No mutation | No mutation | EGFR wild-type |
#4 | 53 | M | No mutation | EGFR E746_A750del (44.2%); TP53 R158L (97.8%) | EGFR E746_A750del (43.6%); TP53 R158L (77.0%) | EGFR E746_A750del (67.4%); TP53 R158L (40.0%) | Not available |
#5 | 73 | F | No mutation | EGFR L858R (34.4%) | ALK G689R (1.2%); EGFR L858R (7.3%) | EGFR L858R (24.1%) | Not available |
#6 | 54 | M | FGFR1 R455H (46.8%) | EGFR E746Valfs (7.3%), P753Rfs (11.4%); FGFR1 R455H (33.4%) | EGFR E746Valfs (5.9%), P753Rfs (6.1%); | Not available | EGFR wild-type |
#7 | 48 | F | EGFR G719S (0.3%) | EGFR L858R (5.3%) | EGFR L858R (2.4%) | Not available | EGFR L858R |
#8 | 60 | F | No mutation | No mutation | No mutation | Not available | Not available |
#9 | 35 | M | EGFR G719S (0.1%) | KRAS Q61L (0.1%); TP53 W53* (85.5%) | TP53 W53* (45.1%) | Not available - | Not available |
#10 | 72 | F | No mutation | EGFR L858R (51.8%) | EGFR L858R (3.8%) | Not available | EGFR L858R |
#11 | 50 | F | No mutation | EGFR L858R (41.1%); TP53 D228Valfs (87.9%) | EGFR L858R (10.7%); TP53 D228Valfs (12.8%) | Not available | EGFR L858R |
#12 | 54 | F | No mutation | TP53 V272 M (77.3%); EGFR E746_A750del (25.1%) | TP53 V272 M (26.1%); EGFR E746_A750del (19.0%) | Not available | EGFR 19del |
#13 | 55 | M | No mutation | EGFR E746_A750del (19.2%) | Not available | EGFR E746_A750del (43.2%) | EGFR 19del |
#14 | 46 | M | No mutation | No mutation | Not available | EGFR E746_A750del (88.0%) | EGFR 19del |
#15 | 60 | M | EGFR T263P (0.19%) | EGFR L858R (52.7%) | Not available | Not available | EGFR L858R |
#16 | 67 | F | No mutation | No mutation | Not available | No mutation | EGFR wide-type |
#17 | 55 | F | No mutation | No mutation | Not available | EGFR E746_A750del (48.0%); TP53 T125 M (60%) | EGFR 19del |
#18 | 69 | M | No mutation | No mutation | Not available | Not available | EGFR wide-type |
#19 | 43 | F | No mutation | Not available | EGFR E746_A750del (1.2%) | Not available | EGFR 19del |
#20 | 48 | F | No mutation | Not available | Not available | No mutation | EGFR wide-type |
#21 | 72 | F | EGFR E746_A750del (17.8%) | Not available | Not available | EGFR E746_A750del (2.7%) | EGFR 19del |
#22 | 49 | F | No mutation | Not available | Not available | EGFR E746_A750del (52.4%) | EGFR 19del |
#23 | 49 | F | TP53 N268 fs* (22.7%) | Not available | Not available | EGFR E746_A750del (34.3%); TP53 N268 fs* (20.8%) | Not available |
#24 | 67 | F | No mutation | Not available | Not available | No mutation | EGFR wide-type |
#25 | 68 | F | EGFR E746_A750del (0.6%) | Not available | Not available | EGFR E746_A750del (88.4%) | EGFR 19del |
#26 | 43 | F | No mutation | Not available | Not available | No mutation | EGFR wide-type |
#27 | 66 | M | No mutation | Not available | Not available | TP53 R280S (38.7%) | EGFR L858R |
#28 | 72 | M | No mutation | Not available | Not available | EGFR L858R (27.6%) | EGFR L858R |
#29 | 71 | F | No mutation | Not available | Not available | No mutation | EGFR 19del |
Total Tests | 29 | 18 | 13 | 19 | 24 | ||