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Table 1 Inter-assay concordance analysis of human MYCN status determined by ddPCR, IHC (N-myc) and FISH in human neuroblastoma

From: Droplet digital PCR as an alternative to FISH for MYCN amplification detection in human neuroblastoma FFPE samples

DD-PCR FISH Amplified FISH Non-amplified Concordance by DDPCR Discordance by DDPCR
 Non-amplified (n = 68) 3 65 (65/65)100% (0/65)0%
 Amplified (n = 11) 11 0 (11/14)79% (3/14)21%
IHC FISH Amplified FISH Non-amplified Concordance by IHC Discordance by IHC
 Negative (n = 64) 3 61 (61/64)95.3% (3/64)4.7%
 Positive (n = 15) 11 4 (11/15)73% (4/15)27%
DD-PCR IHC Positive IHC Negative Concordance by DDPCR Discordance by DDPCR
 Non-amplified (n = 68) 4 64 (64/68)94.1% (4/68)5.9%
 Amplified (n = 11) 11 0 (11/11)100% (0/11)0%
  1. A total of 79 selected cases with known MYCN status (14 amplified and 65 non-amplified) assessed by FISH as a part of clinical diagnosis were included in the analysis. Number of cases and percent measures of concordance in FISH vs ddPCR, IHC vs. FISH, and ddPCR vs. IHC are presented for amplified and non-amplified cases
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