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Table 1 Inter-assay concordance analysis of human MYCN status determined by ddPCR, IHC (N-myc) and FISH in human neuroblastoma

From: Droplet digital PCR as an alternative to FISH for MYCN amplification detection in human neuroblastoma FFPE samples

DD-PCR

FISH Amplified

FISH Non-amplified

Concordance by DDPCR

Discordance by DDPCR

 Non-amplified (n = 68)

3

65

(65/65)100%

(0/65)0%

 Amplified (n = 11)

11

0

(11/14)79%

(3/14)21%

IHC

FISH Amplified

FISH Non-amplified

Concordance by IHC

Discordance by IHC

 Negative (n = 64)

3

61

(61/64)95.3%

(3/64)4.7%

 Positive (n = 15)

11

4

(11/15)73%

(4/15)27%

DD-PCR

IHC Positive

IHC Negative

Concordance by DDPCR

Discordance by DDPCR

 Non-amplified (n = 68)

4

64

(64/68)94.1%

(4/68)5.9%

 Amplified (n = 11)

11

0

(11/11)100%

(0/11)0%

  1. A total of 79 selected cases with known MYCN status (14 amplified and 65 non-amplified) assessed by FISH as a part of clinical diagnosis were included in the analysis. Number of cases and percent measures of concordance in FISH vs ddPCR, IHC vs. FISH, and ddPCR vs. IHC are presented for amplified and non-amplified cases
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BMC Cancer

ISSN: 1471-2407

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